Bio-One Colorado

Navigating the complex landscape of franchise opportunities requires meticulous due diligence, particularly for investors seeking a resilient business model with significant societal impact and strong growth potential. One such compelling opportunity lies within the specialized sector of biohazard and decontamination cleanup services, an industry marked by consistent demand irrespective of economic fluctuations. Bio-One, a leading franchise in this critical field, operates under the deeply ingrained mission of "Help First, Business Second," a philosophy that resonates profoundly with its network of dedicated franchisees and the communities they serve. The foundational story of Bio-One traces back to either 2006, 2007, or 2008, establishing its roots in a vital service niche that addresses sensitive and often urgent needs. While specific founders are not explicitly named in publicly available records, the company's strategic headquarters have been identified across several prominent locations including Greenwood Village, Colorado; Denver, Colorado; and Littleton, Colorado, with one additional source also citing Springville, UT, USA, reflecting a dynamic operational footprint. This robust infrastructure supports a rapidly expanding network that began franchising in 2010. As of September 2023, Bio-One had surpassed 100 locations, extending its essential services across 41 states. Further data points indicate a network of over 100 locations, 130+ locations spanning 45 states, and a total of 140 units, with the 2025 Franchise Disclosure Document (FDD) specifically reporting 137 franchised Bio-One locations operating within the United States. In 2021, the brand boasted over 115 offices in more than 40 states, demonstrating a consistent growth trajectory. The brand’s operations are focused exclusively within the United States, covering 38 states plus Washington D.C., with the Southern region representing the largest concentration of activity with 58 franchise locations. Within this national framework, the Bioone Colorado network stands as a significant operational entity, comprising 68 franchised units, all of which are independently owned and operated, underscoring the brand's commitment to a purely franchised model. This robust presence positions Bioone Colorado as a dominant player in a critical niche, offering essential services within a total addressable market projected to reach substantial valuations, making the Bioone Colorado franchise opportunity a noteworthy consideration for serious investors.

The industry landscape in which Bioone Colorado operates is characterized by its essential nature and impressive growth projections, firmly positioning it within the broader environmental remediation services market. The crime and trauma scene cleanup services market, a specialized segment Bio-One dominates, is anticipated to expand to $390 million by 2027, exhibiting a Compound Annual Growth Rate (CAGR) of 5.2%. This growth is further amplified by the broader environmental remediation services market, which was valued at an estimated USD 1.15 billion in 2025 and is expected to climb to USD 1.21 billion in 2026, with a sustained CAGR of 5.61% projected to reach USD 1.68 billion by 2032. Alternative estimations paint an even larger picture, placing the environmental remediation services market at USD 138.90 billion in 2025, with a trajectory to reach USD 238.21 billion by 2032, driven by a compelling CAGR of 8.0% from 2025. Globally, the environmental remediation market value is forecast to attain USD 210.56 billion by 2030, growing from USD 141.87 billion in 2025 at a CAGR of 8.2% during the forecast period. These figures underscore a robust and expanding market, creating a fertile ground for a specialized service provider like Bioone Colorado. Key consumer trends are instrumental in driving this escalating demand, including a heightened public awareness regarding pathogen transmission risks, coupled with evolving regulatory expectations that mandate stringent cleanup and decontamination protocols. Furthermore, there is an increasing societal focus on developing environmentally friendly industries that prioritize pollution control and ecological balance, which inherently fuels the demand for innovative and compliant remediation solutions. These secular tailwinds significantly benefit the Bioone Colorado franchise, as its services are not only recession-resistant but also increasingly essential due to stringent government regulations concerning hazardous waste treatment and disposal, growing industrial activities, and a more informed public concerned about health issues stemming from contamination. North America plays a dominant role in this market, expected to command a substantial 39.8% market share in 2025, with the oil and gas segment anticipated to generate the strongest demand within the application segment, holding a market share of 34.09% in 2025. The competitive dynamics within this specialized niche, though fragmented in some areas, see Bio-One consistently recognized for its leadership, indicating a strong positioning for its franchisees, including the Bioone Colorado units, within this critical and growing industry.

For prospective investors evaluating the Bioone Colorado franchise opportunity, a detailed understanding of the investment structure is paramount. While specific franchise fee data for Bioone Colorado is not directly available, the national Bio-One brand typically charges an initial franchise fee of $60,000, although an older FDD reference from 2023 indicated $35,000. This $60,000 figure is consistent across more recent sources, providing a benchmark for the category. The total initial investment required to launch a Bioone Colorado franchise is distinctly outlined, ranging from a low of $55,005 to a high of $118,600. This specific range for Bioone Colorado presents a compelling entry point compared to the broader national Bio-One brand's investment ranges, which are quoted between $116,000 and $170,000, or $134,645 to $221,095, or $132,145 to $217,595, and a September 2023 estimate of $110,695 to $164,195. The lower initial investment for Bioone Colorado makes it a potentially more accessible mid-tier franchise opportunity. These investment figures typically encompass a variety of expenditures, which for the national Bio-One brand include the initial franchise fee, a QSP Package costing $39,500, a Technology Startup Fee of $1,595, and an Annual Conference Registration Deposit of $1,000. Operational setup costs are streamlined, with rent and rental improvements ranging from $0 to $600, and furniture, fixtures, and equipment from $0 to $2,000, reflecting the brand's low-overhead model. Essential technology outlays for computer hardware, software, and other electronics are estimated between $800 and $7,800. Comprehensive training is covered by initial training expenses of $1,000 to $2,500, an OSHA Training Fee of $1,500, and OSHA Training Expenses of $1,000 to $2,500. Vehicle-related costs are also factored in, with a business vehicle ranging from $0 to $30,000, business vehicle tax, title, and license from $0 to $1,500, and business vehicle insurance from $150 to $300. Further costs include insurance and professional services ($750 to $4,000), additional funds for three months ($9,000 to $15,000), and working capital ($15,000 to $25,000). For the national Bio-One brand, the minimum liquid capital required is $60,000, with a minimum net worth requirement of either $100,000 or $250,000. Ongoing fees for a Bioone Colorado franchise include a royalty fee of 7.50% of gross sales, aligning with the national brand's structure. The advertising fund, for the national brand, is structured as 2% + 5%, or the greater of $200 per month or 2% of Gross Sales, or a Brand Fund that is the greater of $145 per month or 2% of Gross Sales. Bio-One also extends a 15% discount off the initial franchise fee for eligible veterans and first responders, enhancing accessibility for these deserving individuals. The acquisition of Bio-One by Five Star Franchising in 2021 provides corporate backing and resources, potentially offering franchisees, including those operating Bioone Colorado units, enhanced support and stability within the platform.

The operating model and support structure for a Bioone Colorado franchise are meticulously designed to empower franchisees, ensuring high standards of service delivery and operational efficiency, even for individuals without prior industry experience. A typical day for a Bio-One owner often involves executing a daily marketing plan, a proactive approach crucial for generating leads in this demand-driven service sector. Franchisees are primarily responsible for providing immediate, high-quality decontamination and biohazard cleanup services, a role that demands exceptional discretion, care, and compassion. Owners, such as Matt and Krista Gregg of Bio-One of Colorado, consistently emphasize the importance of compassion, professionalism, and respect in their interactions, often working directly with insurance adjusters to alleviate the burden on clients during distressful times. H. Dale Palmer, owner of Bio-One of Colorado Springs, similarly underlines the critical need for professionalism and empathy in every service call. The business model is inherently lean, characterized by low overhead and a requirement for limited staff or only a few technicians per job, which significantly streamlines operations and reduces labor costs. This approach encourages franchise owners to focus on building a hardworking, dedicated team capable of handling sensitive situations with expertise. Bio-One operates predominantly as a low-overhead, home-based business model, cleverly eliminating the necessity for a traditional brick-and-mortar location or complex buildout. This strategic choice not only reduces fixed costs but also allows for greater flexibility and a faster market entry. The comprehensive training program provided by Bio-One is a cornerstone of its support structure, offering extensive hands-on instruction in biohazard remediation techniques and sensitive trauma-handling protocols. This ensures that every franchisee, including those establishing a Bioone Colorado operation, is fully equipped to perform jobs safely and effectively. The training encompasses 40 hours of combined on-the-job and classroom instruction, covering essential operational, technical, and customer service skills. Recognizing that franchisees may not have prior experience in cleanup or emergency response, Bio-One provides all necessary training, including specific OSHA training, which involves a fee of $1,500 and associated expenses ranging from $1,000 to $2,500. Ongoing corporate support is robust, granting franchisees access to proprietary playbooks that cover critical areas such as staffing, personal protective equipment (PPE) protocols, and risk management. This structured guidance allows for a remarkably fast startup timeline, often enabling new franchisees to commence operations within 45 to 60 days. Franchisees benefit from a dedicated leadership team and a supportive community of fellow owners who collectively prioritize operational excellence. This collaborative environment is further enhanced by access to purchasing co-ops, a suite of marketing tools, and extensive online resources, complemented by invaluable hands-on field experiences. Bio-One’s emphasis on a "systems-driven model" ensures consistency and quality across all units, including every Bioone Colorado location. Territory information, crucial for strategic market penetration, is thoroughly discussed with potential franchisees during the discovery call process, providing clarity on exclusive operating areas. The model is specifically designed for high-resilience leaders, indicating a preference for owner-operators who are deeply engaged in the business's daily functions.

When evaluating the financial performance of a Bioone Colorado franchise, it is critical to note that Item 19 financial performance data, which typically provides detailed revenue and profit figures, is not disclosed in the current Franchise Disclosure Document. This means prospective investors must rely on other indicators and industry benchmarks to assess the potential profitability of the Bioone Colorado franchise opportunity. Despite the absence of Item 19 disclosure, the national Bio-One brand has publicly reported an average revenue per unit of $762,596, offering a valuable insight into the potential earning capabilities within the system. Furthermore, the brand confidently claims to have "high profit margins," a statement that, while not quantified, suggests a favorable cost structure relative to revenue. It is important to distinguish that while companies on the 2021 Inc. 5000 list reported an average median three-year growth rate of 543 percent and median revenue reaching $11.1 million, these figures refer to Inc. 5000 companies generally and do not specifically represent Bio-One's average unit revenue. However, Bio-One's own inclusion on the Inc. 5000 list—ranking No. 2,476 in 2021 and No. 4,365 in 2022—along with its consistent recognition in Entrepreneur magazine's Franchise 500, where it was ranked #381 in 2021 for its 43rd annual list and #1 in Crime Scene Cleaning and Trauma Scene Cleaning for the 42nd list, as well as #1 in Entrepreneur's Best of the Best for Crime-Scene Cleaning in 2021, serve as strong indirect indicators of robust brand performance and market acceptance. These accolades, which the Bioone Colorado units contribute to, suggest that the brand's units are performing strongly within their markets. The significant growth trajectory of the national brand, expanding from over 115 offices in 2021 to 137 franchised units in the USA as of the 2025 FDD, further signals underlying unit-level viability and demand for its services. The inherent nature of Bio-One’s services—essential and recession-resistant—means that demand persists regardless of broader economic factors, providing a stable revenue stream for franchisees, including those operating Bioone Colorado locations. This resilience, coupled with the brand’s strategic positioning in a growing market driven by increasing public awareness and stringent regulations, suggests a favorable environment for generating consistent revenue and achieving the claimed high profit margins, even in the absence of explicit Item 19 data for Bioone Colorado.

The growth trajectory and competitive advantages of the Bioone Colorado franchise are deeply intertwined with the national brand's impressive expansion and strategic market positioning. Bio-One began franchising in 2010 and has since demonstrated significant growth, with its unit count consistently climbing. The network has expanded beyond 100 locations, reaching over 130 locations in 45 states, with a reported 140 total units and specifically 137 franchised Bio-One locations across the USA as of the 2025 Franchise Disclosure Document. In 2021, the company operated over 115 offices in more than 40 states, showcasing a sustained upward trend in market penetration. The Bioone Colorado network, with its 68 franchised units, represents a substantial portion of this national footprint, reflecting the brand's successful expansion strategy. Recent corporate developments include the acquisition of Bio-One by Five Star Franchising in 2021, a strategic move that integrated the brand into a larger platform, providing enhanced resources and support for continued expansion. Leadership changes have also occurred, with Brian Brinegar serving as President of Bio-One Inc. in 2021, followed by Sandi Ellis taking on the role of President in 2022, indicating a dynamic and evolving corporate structure focused on driving growth. The competitive moat for Bio-One is multifaceted, built upon its foundational "Help First, Business Second" philosophy, which fosters deep trust and loyalty within communities. The comprehensive training and systems-driven model ensure consistent, high-quality service delivery across all locations, including every Bioone Colorado operation. The brand's low-overhead, home-based business model is a distinct advantage, minimizing fixed costs and maximizing operational flexibility. Furthermore, Bio-One has cultivated strong brand recognition through features on popular shows like A&E's Hoarders and 60 Minutes, and its membership in the FBI National Academy Associates, lending significant credibility and visibility. The brand's consistent receipt of prestigious awards further solidifies its competitive standing: it was ranked number one in Crime Scene Cleaning and Trauma Scene Cleaning in Entrepreneur magazine's 42nd Franchise 500 list, secured #381 in the 43rd annual Franchise 500® in 2021 (marking its sixth consecutive year on the list), and was named #1 in Entrepreneur's Best of the Best for Crime-Scene Cleaning in 2021. Its inclusion on the Inc. 5000 list (No. 2,476 in 2021 and No. 4,365 in 2022) and recognition in FranServe's Fran-Tastic 500 highlight its rapid growth and strong performance. The brand is actively adapting to current market conditions by emphasizing its essential, recession-resistant services and leveraging its systems-driven model for efficiency. Bio-One is explicitly "well-positioned and supported for rapid expansion and growth across North America," actively seeking entrepreneurs to launch new businesses, indicating robust future expansion plans for the Bioone Colorado and wider network.

The ideal franchisee for a Bioone Colorado franchise is a high-resilience leader who is deeply committed to serving their community with compassion and professionalism. While prior experience in cleanup or emergency response is not a prerequisite, given Bio-One's comprehensive training program, a strong managerial background and an understanding of operational excellence are highly beneficial. Franchisees consistently express that the rewarding nature of the work, coupled with the robust support received from the franchisor and fellow owners, significantly outweighs other considerations. Owners like Matt and Krista Gregg of Bio-One of Colorado exemplify the compassionate, professional, and respectful approach that is central to the brand's mission, working diligently with clients and insurance adjusters to ease burdens during difficult times. H. Dale Palmer, another owner of Bio-One of Colorado Springs, similarly embodies the professionalism and empathy that defines a successful Bio-One operator. The business model, with its low overhead and limited staffing requirements, is particularly suited for owner-operators who wish to be actively involved in their business while building a dedicated team. Although specific multi-unit requirements are not explicitly detailed, the brand's rapid expansion across the country suggests ample opportunity for growth-oriented franchisees to develop multiple Bioone Colorado territories. Current operations span 38 states plus Washington D.C., with a significant concentration of 58 locations in the South, indicating diverse market performance potential. The Bioone Colorado network itself, comprising 68 franchised units, demonstrates the brand's strong presence and viability within the state. Available territories are discussed in detail with potential franchisees during the discovery call process, ensuring strategic placement and market exclusivity. The timeline from signing the franchise agreement to opening for business is remarkably fast, often within 45 to 60 days, thanks to the streamlined, home-based model and comprehensive startup support. Franchisees consistently praise the network of franchise owners as "fantastic" and appreciate the "dramatic change in the direction of the organization, in a positive way," underscoring a supportive and evolving corporate culture. The sentiment that "we not only help the community, but we help each other" further illustrates the strong sense of camaraderie and mutual support within the Bio-One family, including the Bioone Colorado community. While the specific term length for the franchise agreement is not available, these positive testimonials and the structured support system contribute to a strong foundation for long-term engagement and success.

For franchise investors seeking a unique opportunity within an essential, recession-resistant industry, the Bioone Colorado franchise warrants serious due diligence. The brand's unwavering commitment to its "Help First, Business Second" mission, coupled with a proven, low-overhead business model, positions it favorably within the specialized niche of biohazard and trauma scene cleanup. With the crime and trauma scene cleanup market projected to grow to $390 million by 2027 at a CAGR of 5.2%, and the broader environmental remediation services market expected to reach USD 238.21 billion by 2032 with an 8.0% CAGR, the Bioone Colorado franchise is situated within a sector driven by sustained demand, stringent regulations, and heightened public awareness. The significant national unit growth, with 137 franchised locations across the USA as of the 2025 FDD, and the specific Bioone Colorado network comprising 68 franchised units, underscore the brand's operational scalability and market acceptance. Despite Item 19 financial performance data not being disclosed in the current FDD, the national brand's reported average revenue of $762,596 and claims of "high profit margins," alongside its strong FPI Score of 76 (Strong), provide compelling indicators of potential profitability and unit-level success. The comprehensive training, rapid 45-60 day startup timeline, and robust ongoing support from a dedicated leadership team and a collaborative franchisee community, including local owners like Matt and Krista Gregg of Bio-One of Colorado, mitigate many of the typical risks associated with new business ventures. This opportunity offers not just financial growth but also the profound personal reward of providing critical services to